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KMID : 0368420140570010043
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Journal of Plant Biology
2014 Volume.57 No. 1 p.43 ~ p.47
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Knockdown expression of the B-type cyclin gene Orysa;CycB1;1 leads to triploid rice
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Guo Jing
Wang Fang
Zhang Xian Sheng
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Abstract
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B-type cyclins play key roles in the mitotic cell cycle and endoreduplication. Our previous study showed that knockdown of Orysa;CycB1;1 resulted in abnormal seeds and low seed production. To investigate the functions of B-type cyclins in plant reproductive growth, we analyzed the ploidy of the abnormal P ubi ::CycB1;1 RNAi embryo. The results showed that the abnormal P ubi ::CycB1;1 RNAi embryo was triploid. The triploid seedlings had larger reproductive organs than those of control plants, and were abortive. Semi-quantitative RT-PCR and in situ hybridization analyses showed that Orysa;CycB1;1 transcripts were localized in cells of anthers and ovaries. This indicated that Orysa;CycB1;1 is critical for reproductive growth and might be involved in gametogenesis in rice. The results of this study increase our understanding of the functions of B-type cyclins in rice reproductive growth.
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KEYWORD
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Embryo, Orysa, CycB1, 1, Rice, Triploid
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